TABLE 1.

Binding affinities of PICK1 ligand peptide

The K_i values for the listed C-terminal peptides were calculated from FP competition assays on purified full-length PICK1 in solution using Oregon Green-labeled DAT C13 peptide as tracer (32). Data are average of 6–12 determinations (means ± S.E. interval).

Ligand peptide	Sequence	Type	Ki (μm), mean ± S.E.
PKCα	QFVHPILQSAV	I	14.1 [13.4; 14.9]
Aquaporin 2	SPQSLPRGSKA	I	90 [71; 115]
GLT1b	PFPFLDIETCI	I	6.7 [6.3; 7.3]
GluA2	YNVYGIESVKI	II	7.2 [6.6; 7.7]
DAT	RQFTLRHWLKV	II	0.8 [0.7; 0.9]
Ephrin B1	PQSPANIYYKV	II	9.4 [7.3; 12.2]
HER2	NPEYLGLDVPV	II	4.6 [4.1; 5.2]
Aquaporin 9	NLEKHELSVIM	II	12.2 [11.4; 13.1]
PKMζ	NPLLLSAEESV	III	39 [30; 52]
mGluR7B	SVTWYTIPPTV	?	23 [19; 27]
AE1	RDEYDEVAMPV	?	6.1 [5.3; 7.0]
Arf1 GTPase	DWLSNQLRNQK	?	No affinity
ASIC1a	PARGTFEDFTC	?	6.3 [5.5; 7.2]
UNC5H1	AGLFTVSEAEC	?	2.8 [2.5; 3.1]
NCS-1	VQALSLYDGLV	?	21 [17; 25]