TABLE 5.

Binding affinities for C5 ligand peptides

K_i values for the listed pentameric peptides were calculated from FP competition assays on purified full-length PICK1 WT in solution using Oregon Green-labeled DAT C13 peptide as tracer (32). Data are average of nine determinations (means ± S.E. interval).

Ligand peptide	C5 sequence	Ki (μm), mean ± S.E.	Difference from C11
PKCα	LQSAV	>100	− >10
GluA2	ESVKI	13.3 [12.4; 14.2]	− 1.8
HER2	LDVPV	28 [27; 29]	− 6.1
ASIC1a	EDFTC	11.7 [11.0; 12.4]	− 1.9
UNC5H1	SEAEC	6.5 [6.1; 7.1]	− 2.3
DAT	HWLKV	1.8 [1.4; 2.3]	− 2.3
GLT1b	IETCI	28 [26; 29]	− 4.2