TABLE 6.

Binding affinities for PKCα mutated peptides

K_i values for the listed peptides were calculated from FP competition assays on purified full-length PICK1 WT in solution using Oregon Green-labeled DAT C13 peptide as tracer (32). Data are averages of six determinations (means ± S.E. interval).

Ligand peptide	Sequence	Ki (μm), mean ± S.E.
PKCα	QFVHPILQSAV	14.1 [13.4; 14.9]a
PKCα Pro P−6 → Ala	QFVHAILQSAV	243 [186; 317]
PKCα His P−7 → Ala	QFVAPILQSAV	842 [594; 1194]

^a These are the same values as in Table 1.