FIGURE 1.

SNX17 binds directly to KRIT1 NPXF2. A, schematic diagram of SNX17 and KRIT1 proteins. In this study, we demonstrate that the F3 lobe of the FERM domain of SNX17 (green) directly binds KRIT1 NPXF2 (orange). B, sequence alignment of human KRIT1 NPX(Y/F) motifs. The position and numbering of the NPX(Y/F) residues is shown. C, endogenous SNX17 from HEK293T cells binds KRIT1-NPXF2 in GST pulldown assays. The cytoplasmic tail of P-selectin (p-sel) is included as a positive control, and GST alone is included as a negative control. Top panel, bound SNX17 is visualized by anti-SNX17 immunoblot (IB). Bottom panel, equal GST loading is shown by Ponceau staining. D, purified recombinant SNX17 PX-FERM binds directly to KRIT1 NPXF2 in a similar GST pulldown experiment from C. Proteins are visualized by Coomassie Blue staining.