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. 2014 Aug 19;2:25. doi: 10.1186/2051-1426-2-25

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Copyright © 2014 Beard et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Reverse-phase protein array. Total proteins were extracted from frozen tissues and applied to Multi-Spot™ plates (see Methods). Anti-CSPG4 (TP41.2) and anti-Actin antibodies were applied and following incubation and wash, detected with SULFO-TAG™ antibodies. Signal was normalized to actin expression and is expressed as relatively fold over background (water). The horizontal dashed line is the threshold antigen detection value set as the background mean plus one standard deviation. Each bar is protein from a different donor from the indicated tissues.