Figure 5.

TLR2 signaling is required for maximal cytokine and chemokine production in the lung after LVS clpB inoculation. B6 or TLR2 KO mice were intranasally inoculated with 5 × 10⁴ CFU LVS clpB or were left naive. Three days post-inoculation, mice were sacrificed and BALF was collected, and cytokine and chemokine concentrations were determined using a Luminex-based assay. For each pair of groups, a fold-changed was determined based on the average cytokine or chemokine concentrations. Data are combined from two independent experiments. n = 7–12 mice/group. Statistical significance was determined using ANOVA with Tukey’s post-test on the absolute concentration of each analyte.