TABLE 3.

MICs of antimicrobial agents for the parental strain, transconjugant, and transformant

β-Lactama	MIC (μg/ml)
	K. pneumoniae KG525	E. coli CSH-2(pKGC525)b	E. coli CSH-2	E. coli XL1-Blue(pKGB525)c	E. coli XL1-Blue(pBCSK+)
Ampicillin	>128	>128	4	>128	4
Ampicillin + sulbactam	>128	2	2	2	2
Amoxicillin	>128	>128	8	>128	4
Amoxicillin + clavulanate	>128	32	4	32	4
Piperacillin	128	16	1	16	1
Piperacillin + tazobactam	128	0.5	1	0.5	0.5
Cefotaxime	64	2	0.13	2	0.13
Cefotaxime + clavulanate	8	0.06	0.06	0.06	0.06
Cefotaxime + sulbactam	32	0.06	0.06	0.06	0.06
Cefotaxime + tazobactam	64	0.06	0.06	0.06	0.06
Ceftazidime	>1,024	128	0.13	128	0.13
Ceftazidime + clavulanate	256	4	0.13	4	0.06
Ceftazidime + sulbactam	>128	0.25	0.13	0.5	0.13
Ceftazidime + tazobactam	>128	0.5	0.13	0.5	0.13
Cephaloridine	>128	16	2	16	2
Cefminox	8	0.5	0.5	1	0.5
Moxalactam	4	0.25	0.13	0.5	0.13
Cefpirome	>128	1	0.06	2	0.06
Cefepime	16	0.13	0.06	0.25	0.06
Aztreonam	64	4	0.06	4	0.06
Imipenem	0.25	0.5	0.25	0.13	0.13
Gentamicin	2	0.13	0.13	<0.06	<0.06
Amikacin	64	2	0.25	4	0.25
Levofloxacin	<0.06	0.13	0.13	<0.06	<0.06
Ciprofloxacin	<0.06	<0.06	<0.06	<0.06	<0.06

^aClavulanate, tazobactam, and sulbactam were used at a fixed concentration of 4 μg/ml each.

^bpKGC525 is a resident plasmid found in K. pneumoniae strain KG525, and it carries the bla_GES-3 gene.

^cpKGB525 is a recombinant plasmid that carries a 6.7-kb BamHI insert that mediates the bla_GES-3 gene.