FIG. 2.

Effect of 3(2H)-isoflavene on RNA synthesis of PV2 Sabin, R2/01, and D6/01 variants. (A) HeLa cells monolayers at 60% confluency were transfected with purified viral RNA from PV2 Sabin and viral variants in the presence of 0.5 mg of DEAE-dextran per ml. Following incubation for 6 h in the presence or absence of the drug (20 μM), RNAs were extracted, adsorbed on filter paper (10 μg), and hybridized with a probe (specific for PV2 nucleotides 1500 to 1940). After autoradiography, RNAs were quantified as described in Materials and Methods. (B) HeLa cell monolayers were infected with viral variants and the reference Sabin PV2 strain at MOIs of 10 and incubated at 37°C in the absence or presence of the antiviral compound (20 μM). After a single step of viral replication (6 h postinfection), total RNAs were extracted and adsorbed (5 μg) on filter paper. Hybridization was performed as described for panel A. Solid bars, without antiviral compound; shaded bars, with antiviral compound.