Fig. 1.
Relative binding sensorgram of Biacore surface plasmon resonance analysis of 100 nM Anti-HER2-ONH2 3 and 100 nM FBO-Anti-HER2 4 against chimeric HER2/Fc protein used as a ligand. Full kinetic analysis was performed on both conjugates at concentrations ranging from 0 nM to 100 nM to determine effect of modification of the conjugate upon modification on binding affinity.