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. Author manuscript; available in PMC: 2014 Sep 5.
Published in final edited form as: Nat Protoc. 2014 Jan 9;9(2):263–293. doi: 10.1038/nprot.2014.012

Fig. 5. The positional profiles of each type of mutation relative to 5′ end of reads (step 105).

Fig. 5

The y-axis shows the percentage of unique tags with a particular type of mutation in each position. (a) deletions; (b) substitutions; (c) insertions. The U shaped distribution for substitutions and deletions is characteristic of the positional sequencing error profile of the Illumina platform, while a higher rate of deletion in the middle (peaked at around positions 5-15) is a signature of protection from RNase digestion by the RNABP binding footprint.