| Component | Amt. per reaction | Amt. in master mix (for 8.5 reactions) |
| Accuprime Pfx Supermix | 27 μL | 229.5 μL |
| DP5 primer (20 μM stock) | 0.5 μL | 4.25 μL |
| DP3 primer (20 μM stock). | 0.5 μL | 4.25 μL |
| RT mix | 2.5 μL | 20 μL (whole mix) |
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