| Component | Amt. per reaction | Amt. in master mix (for 2.5 reactions) |
| Accuprime Pfx Supermix | 27 μL | 67.5 μL |
| DP5 primer (20 μM stock) | 0.5 μL | 1.25 μL |
| DP3 primer (20 μM stock). | 0.5 μL | 1.25 μL |
| RT mix | 2.5 μL | 6.25 μL |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.