Mapping Argonaute and conventional RNA-binding protein interactions with RNA at single-nucleotide resolution using HITS-CLIP and CIMS analysis

. Author manuscript; available in PMC: 2014 Sep 5.

Published in final edited form as: Nat Protoc. 2014 Jan 9;9(2):263–293. doi: 10.1038/nprot.2014.012

Component	Amt. per reaction	Amt. in master mix (for 4.5 reactions)
Accuprime Pfx Supermix	27 μL	121.5 μL
SP5 primer (20 μM stock)	0.5 μL	2.25 μL
SP3 primer (20 μM stock).	0.5 μL	2.25 μL
Purified PCR product from Step 77	2 μL	9 μL