Mapping Argonaute and conventional RNA-binding protein interactions with RNA at single-nucleotide resolution using HITS-CLIP and CIMS analysis

. Author manuscript; available in PMC: 2014 Sep 5.

Published in final edited form as: Nat Protoc. 2014 Jan 9;9(2):263–293. doi: 10.1038/nprot.2014.012

RNase-free water	47 μL
T4 RNA Ligase Buffer, 10×	8 μL
BSA (1mg/mL)	8 μL
10 mM ATP	8 μL
³²P-labeled RL3 linker (prepared as in Box 1)	5 μL
T4 RNA Ligase I	2 μL
Optional: RNAsin Plus (Promega).	2 μL