RNase-free water	44 μL
T4 RNA Ligase Buffer, 10×	8 μL
BSA (0.2 mg/mL)	8 μL
10 mM ATP	8 μL
RL3 linker (IMPORTANT: with 5′-phosphate) @ 20 μM	8 μL
T4 RNA Ligase I (Fermentas)	2 μL
Optional: RNAsin Plus (Promega).	2 μL