Mapping Argonaute and conventional RNA-binding protein interactions with RNA at single-nucleotide resolution using HITS-CLIP and CIMS analysis

. Author manuscript; available in PMC: 2014 Sep 5.

Published in final edited form as: Nat Protoc. 2014 Jan 9;9(2):263–293. doi: 10.1038/nprot.2014.012

T4 RNA Ligase Buffer, 10×	1 μL
BSA (0.2 mg/mL, supplied with enzyme)	1 μL
10 mM ATP	1 μL
RL5 or RL5D linker @ 20 μM	1 μL
T4 RNA Ligase I (Fermentas)	0.1 μL