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. 2012 Feb 23;5(1):39–42. doi: 10.1111/j.1752-8062.2011.00376.x

Figure 4.

Figure 4

Expression of HIF‐1a in C4‐2 xenograft tumor downregulated by digoxin. Tumor tissue was frozen at the time of sacrifice to test levels of HIF‐1a by (A) western blot and (B) real‐time PCR. Western blot shows three samples of either control‐ (‐c) or digoxin (‐d)‐treated mice. HIF‐1a protein is downregulated in three digoxin‐treated tumors. Real‐time PCR represents data from three mice in the control‐treated group and six mice in the digoxin‐treated group. Gene expression is relative to GAPDH. Difference seen in HIF‐1a is statistically significant (*p<0.05).