FIGURE 4.

Role of UMSCs on macrophage polarization. The ability of the UMSCs to modulate polarization of macrophages was evaluated. UMSCs were seeded in a transwell insert with a 0.44-μm pore and treated or not with Chase AC+B or Hylase. Inflammatory cells were seeded in a micro-plate and placed in co-culture with the UMSCs seeded in transwell inserts. A and B, morphology of the macrophages (F4/80⁺ cells) was analyzed (green), and nucleus was stained with DAPI (blue). C, numbers of F4/80⁺TNFα⁺ cells were counted to evaluate the M1 phenotype. D, numbers of F4/80⁺IL10⁺ cells were counted to evaluate the M2 phenotype. E, IRF5 staining (red) of macrophages is shown, and F4/80⁺ cells are shown in green. Scale bars, B and E are 20 μm. *, <0.05 compared with macrophages alone.