FIGURE 5.

Role of UMSCs on the maturation of T-regulatory cells. The ability of the UMSCs to activate T-regulatory cells was evaluated. UMSCs were seeded in a transwell insert with a 0.44-μm pore and treated or not with Chase ABC. Inflammatory cells were seeded in a micro-plate and placed in co-culture with the UMSCs. A, numbers of CD44⁺ cells were counted. B, numbers of F4/80⁻IL10⁺ cells were counted. C and D, numbers of CD25⁺ cells were analyzed and counted, respectively. E, morphology of the macrophages (CD11b⁺ cells) was analyzed in the presence of UMSCs with neutralizing anti-IL10 or anti-TGFβ antibodies. Scale bar, E is 20 μm. F, numbers of stellate CD11b⁺ cells were counted to evaluate the M1 phenotype. *, <0.05 compared with control.