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UMSCs have rich glycocalyces. UMSCs were treated or not with Chase ABC or Hylase and placed in co-culture with inflammatory cells seeded in a transwell insert with a 0.44-μm pore. A, expression and localization of TSG6 and CD44 were evaluated by immunocytochemistry. B, expression of CD44 was further evaluated by Western blotting. C, pentraxin 3 expression and localization were analyzed by immunocytochemistry.
