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NKCC2-VAMP2 co-localization at the apical surface of TALs. A, diagram showing the exocytic process that allows immunodetection of surface NKCC2 and surface VAMP2-eGFP at the apical membrane of cultured TAL cells before fixation. B, apical distribution of surface NKCC2 (red) and surface VAMP2 (green) in cultured TALs. Apical surface NKCC2 was distributed in clusters. 45 ± 7% of NKCC2 clusters also contained VAMP2 (co-localizing pixels) (n = 5).
