Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jul 9;289(34):23951–23962. doi: 10.1074/jbc.M114.589333

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version full access.

Creative Commons Attribution Unported License applies to Author Choice Articles

PMC Copyright notice

FIGURE 7. — VAMP2 silencing does not affect NKCC2 phosphorylation at Ser-126 in TALs. NKCC2 phosphorylation at Ser-126 (pSer-126) was measured with an anti-phospho antibody. Baseline NKCC2 phosphorylation at Ser-126 was undetectable and was not affected by VAMP2 silencing. Stimulation with 100 μm db-cAMP for 20 min enhanced NKCC2 Ser-126 phosphorylation 69 ± 14-fold in control TALs and 73 ± 24-fold in VAMP2-shRNA-transduced TALs. The degree of stimulation by db-cAMP was not statistically significant. Values are expressed as mean -fold increase from vehicle/scrambled shRNA, and error bars represent S.E. *, p < 0.05 versus vehicle (n = 4).