FIGURE 1.
Cell adherence induced by alanine-substituted RasGAP317–326 peptides. A and B, U2OS cells were cultured overnight and then treated for 8 h with 20 μm TAT or 20 μm of the indicated TAT-coupled peptides or left untreated. The cells were then subjected to trypsin-mediated detachment assays. The images shown in panel A correspond to 20 μm peptide-treated cells (n = 4 independent experiments). Scale bar: 200 μm for all images. w/o trypsin, without trypsin. B, dose-response quantitation (n = 4 independent experiments). C, alignment of the p120 RasGAP317–326 sequences from different species. The amino acids conserved during evolution are shaded in gray. Drosoph., Drosophila.