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NOE connectivities detected in 3 by which the linkages of sugars and P-Etn residues were assigned. Some of the anomeric protons (e.g. H-1 of l-Rha and ManII) show NOE cross-peaks (Table 2) not only to the substituting but also to the neighboring sugar protons to which they are bound, indicating a rather twisted conformation of the branched oligosaccharide. The fatty acid residues attached to the lipid A backbone (R3′, R2″-R2′, R3, and R2) are abbreviated for clarity.
