Skip to main content
. 2004 Jun;48(6):1974–1982. doi: 10.1128/AAC.48.6.1974-1982.2004

FIG. 5.

FIG. 5.

Effects of MXF on LPS-induced activation of NF-κB. (A) THP-1 cells were preincubated in serum-free medium in the absence or presence of MXF and then treated with LPS for 1 h. Nuclear extracts were prepared and assayed for NF-κB by EMSA on a 5% polyacrylamide gel with a double-stranded oligonucleotide containing the NF-κB consensus sequence. Binding competition assays were performed with a 100-fold excess of unlabeled NF-κB oligonucleotide as the competitor (cold NF-κB). (B) Bands were quantified by optical densitometry. (C) Expression of p65 NF-κB protein in LPS-stimulated cells. The Western blot (WB) illustrates the expression of the p65 NF-κB protein in nuclear extracts from cells exposed to LPS and preincubated in the absence or presence of MXF. Representatives of the blots from two experiments are shown.