Comparison of PS I supercomplexes in wild-type and PPD1 RNAi mutant thylakoids.
A, unstained and Coomassie-stained BN-polyacrylamide gels of digitonin-solubilized thylakoid membranes from wild type and PPD1 RNAi mutant (Group IV). B, silver-stained two-dimensional (BN-LiDS) PAGE of digitonin-solubilized thylakoid membranes from wild type and PPD1 RNAi mutant (Group IV). The truncated BN-PAGE dimension, corresponding to molecular mass ∼250 kDa and above, is shown for visual reference, and complexes are numbered as follows: 1, PS I-LHC I-LHC II; 2, PS I-LHC I; 3, ATP synthase; 4, PS I. Cyt, cytochrome.