FIGURE 3.
Bcl-xL mediates protection from ER stress- and differentiation-induced apoptosis in Bcl1 cells. A, Bcl1 cells were pretreated with tunicamycin (Tm) for 5 h prior to treatment with IL-5, LPS, or a combination of both for 24 h with or without 600 nm ABT-737. Apoptosis was measured using annexin V and propidium iodide staining. B, Bcl1 cells were treated with IL-5 and LPS for 96 h. Samples were treated with varying doses of ABT-737 for the final 24 h prior to collection, and apoptosis was measured by annexin V and propidium iodide staining. C, Bcl-1 cells were treated with IL-5 and LPS with or without 600 nm ABT-737 for 72 h. Immunoprecipitation (IP) was performed on lysates prepared from individual treatments. IB, immunoblot. Data are presented as the mean ± S.E. of three independent experiments (*, p < 0.05, **, p < 0.01, ***, p < 0.001).