FIGURE 6.
Knockdown of CHOP abrogates differentiation induced ABT-737 sensitivity. Bcl1 cells infected with shATF4, shCHOP, or pLKO1 vector control lentivirus were treated with IL-5 and LPS for 72 h. 600 nm ABT-737 was added 24 h prior to collection. A–D, apoptosis was measured at 72 h. Western blots were prepared at 72 h (representative experiments in C and D). Data are presented as four independent experiments. (*, p < 0.05, **, p < 0.01). E and F, Bcl1 cells stably infected with shBcl-xL or shGFP lentivirus were untreated or treated with IL-5 and LPS for 72 h. ABT-737 (600 nm) or ABT-199 (600 nm) was added 24 h prior to collection. E, Western blots were prepared at 72- and 96-h time points to verify knockdown. F, apoptosis was measured at 72 h. Data in F are presented as the mean ± S.E. of three independent experiments. (*, p < 0.05, **, p < 0.01).