FIGURE 6.

Correlation analysis of functional affinity estimates from [Ca²⁺]_i mobilization experiments with affinity estimates from radioligand binding experiments for TBPB (A), VCP794 (B), 77-LH-281 (C), and xanomeline (D). Linear regression curves depicted are the line of best fit (solid line) and the 95% confidence bands (dotted line curve). For reference, the WT receptor value is shown as an open symbol, in addition to the line of unity with radioligand binding affinity estimates (dashed line). Outliers, located outside the 95% confidence bands, are labeled with their respective mutations.