FIGURE 9.

Effects of mutations on agonist affinities estimated from pERK1/2 experiments. Bars represent the change in functional affinity estimates (pK_A), compared with the wild-type M₁ mAChR, of agonists 77-LH-28-1 (A), TBPB (B), and VCP794 (C). In cases where an estimate could not be determined for an agonist at a mutation, the x axis label is shown faded. Estimates for ACh and mutations Y82A, L102A, D105E, and Y381A were not determined and as a consequence are not included. *, significant change in affinity relative to wild-type receptor, p < 0.05, one-way ANOVA with Dunnett's post-test.