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. 2014 Jul 3;289(34):23332–23342. doi: 10.1074/jbc.M113.526343

FIGURE 3.

FIGURE 3.

Knockdown of hepatic Dec1 in C57BL/6J mice increases hepatic and serum TG levels. Adenoviruses expressing Dec1-specific shRNA (Ad-shDEC1) or a scrambled shRNA (AdshScram), as a control, were injected into normal C57BL/6J mice to interfere endogenous Dec1 expression. Mice fasted for 6 h were sacrificed at 7 days after adenovirus injection. All samples were collected at 16:00 for further analysis. A, real-time PCR analysis showing the hepatic mRNA level of Dec1 in mice injected with the indicated adenovirus. B and C, biochemical analysis showing hepatic TG (B) content and serum TG (C) content in C57BL/6J mice treated with the indicated adenovirus. Hepatic or serum TG contents were measured by an enzymatic assay. The data shown are means ± S.D. (error bars) (individual points refer to each sample) and were compared between groups by a two-tailed unpaired Student's t test. *, p < 0.05.