FIGURE 5.

Dec1 regulates Srebp-1c gene expression and TG synthesis in mouse primary hepatocytes. Primary hepatocytes were isolated and treated with the indicated adenovirus and subsequently exposed to 5 μm TO901317 for 48 h. Then cells were used for further analysis. A, TG contents in the primary hepatocytes treated as described above. B, real-time PCR analysis showing mRNA levels of genes involved in lipid synthesis in primary hepatocytes treated as in A. C, TG contents in primary hepatocytes infected with the indicated adenovirus and then cultured in completed medium for 48 h before cells were harvested. D, real-time PCR analysis showing mRNA levels of Dec1 and genes involved in lipid synthesis in primary hepatocytes treated as in C. The data shown are the means ± S.D. (error bars) and were compared among groups by a one-way analysis of variance followed by a Fisher least significant difference test. *, p < 0.05.