TABLE 1.

Strains, plasmids, and primers used in this study

	Description/genome/sequence	Source
E. coli strains
DH5α	F− endA1 glnV44 thi-1 recA1 relA1 gyrA96 deoR nupG Φ80dlacZΔM15Δ (lacZYA-argF)U169, hsdR17 (rK− mK+), λ−	Invitrogen
BL21(DE3)	F− ompT gal dcm lon hsdSB(rB− mB−) λ(DE3) pLysS(cmR)a	New England Biolabs
W3110	rph-1IN (rrnD-rrnE)	Coli Genetic Stock Centre
ΔzapA	W3110, ΔzapA	This work
Plasmids
pEJR005b	Modified pET28A (no histidine tag) with WT ftsZ from E. coli	This work
pEJR029b	pBAD24 with WT His6-zapA from E. coli	This work
pEJR029b_D22A	pBAD24 with D22A His6-zapA from E. coli	This work
pEJR029b_R24A	pBAD24 with R24A His6-zapA from E. coli	This work
pEJR029b_N28A	pBAD24 with N28A His6-zapA from E. coli	This work
pEJR029b_D32A	pBAD24 with D32A His6-zapA from E. coli	This work
pEJR029b_D33A	pBAD24 with D33A His6-zapA from E. coli	This work
pEJR029b_D33K	pBAD24 with D33K His6-zapA from E. coli	This work
pEJR029b_R46A	pBAD24 with R46A His6-zapA from E. coli	This work
Primers for mutagenesis
D22A_F	5′-AACTGCCCGCCTGCCCAAAGGGATGCG-3′	This work
D22A_R	5′-CGCATCCCTTTGGGCAGGCGGGCAGTT-3′	This work
R24A_F	5′-GAACTGCCCGCCTGACCAAGCGGATGCGTTGAATC-3′	This work
R24A_R	5′-GATTCAACGCATCCGCTTGGTCAGGCGGGCAGTTC-3′	This work
N28A_F	5′-CCAAAGGGATGCGTTGGCTCAGGCAGCGGACGAT-3′	This work
N28A_R	5′-ATCGTCCGCTGCCTGAGCCAACGCATCCCTTTGG-3′	This work
D32A_F	5′-TGAATCAGGCAGCGGCCGATCTGAACCAACG-3′	This work
D32A_R	5′-CGTTGGTTCAGATCGGCCGCTGCCTGATTCA-3′	This work
D33A_F	5′-CAGGCAGCGGACGCTCTGAACCAACGG-3′	This work
D33A_R	5′-CCGTTGGTTCAGAGCGTCCGCTGCCTG-3′	This work
D33K_F	5′-GAATCAGGCAGCGGACAAGCTGAACCAACGGTTGC-3′	This work
D33K_R	5′-GCAACCGTTGGTTCAGCTTGTCCGCTGCCTGATTC	This work
R46A_F	5′-GGTTGCAAGATCTGAAAGAACGCACTGCAGTCACAAATACTGAA-3′	This work
R46A_R	5′-TTCAGTATTTGTGACTGCAGTGCGTTCTTTCAGATCTTGCAACC-3′	This work

^a cm^R, chloramphenicol resistance.