TABLE 3.
Summary of interactions for ZapA variants with FtsZ filaments
| ZapA variant | EC50a | Max Sed.b | Filaments/bundlec | Cell lengthd |
|---|---|---|---|---|
| μm | μm | |||
| WT | 2.22 | 0.87 | 9.86 ± 0.76 | 2.65 ± 0.06 |
| D22A | 2.67 | 0.93 | 7.33 ± 0.49 | 2.81 ± 0.08 |
| R24A | 4.48 | 0.94 | 7.13 ± 0.76 | 2.96 ± 0.15 |
| N28A | 8.77 | 0.75 | 5.21 ± 0.19 | 3.77 ± 0.25 |
| D32A | 4.86 | 0.71 | 5.73 ± 0.29 | 3.74 ± 0.17 |
| D33A | 8.74 | 0.53 | 5.75 ± 0.38 | 3.66 ± 0.17 |
| D33K | 7.05 | 0.78 | 5.46 ± 0.29 | 3.70 ± 0.10 |
| R46A | 7.61 | 0.74 | NAe | 3.87 ± 0.20 |
a EC50, concentration of ZapA needed to sediment 50% of FtsZ in each reaction.
b Max Sed., maximum proportion of FtsZ pelleted by each ZapA variant in the sedimentation assays.
c Determined from transmission electron micrographs (means ± S.E.).
d Values were measured from transmission electron micrographs (means ± S.E.). The mean cell length of ΔzapA E. coli is 3.52 μm.
e NA, data not available because no bundles were observed in transmission electron micrographs.