Skip to main content
. 2014 Jul 7;289(34):23796–23808. doi: 10.1074/jbc.M114.577387

FIGURE 1.

FIGURE 1.

Low iron diet increases liver miR-130a in mice. A, liver miR-130a, Rnu5g, and miR-103 were measured by qRT-PCR in 12-week-old male and female 129S6/SvEvTac mice after receiving a control diet (Ctrl) or a low iron diet (Low Iron) for 5 weeks (n = 5–6 per group). B–D, liver tissue from 9-week-old female C57BL/6 mice (n = 8 per group) treated with a control (Ctrl) or a low iron diet (Low Iron) for 5 weeks were analyzed for miR-130a, Rnu5g, and miR-130a by qRT-PCR (B), p-Smad1/5/8 and total Smad1 relative to actin protein expression by immunoblot (IB) and chemiluminescence quantification (C), and Bmp6, Id1, and Hamp relative to Rpl19 mRNA expression by qRT-PCR (D). Results are reported as mean ± S.E. of the fold change relative to the control group. *, p < 0.05; **, p < 0.01; ***, p < 0.001.