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. 2014 Jul 7;289(34):23796–23808. doi: 10.1074/jbc.M114.577387

FIGURE 5.

FIGURE 5.

miR-130a mimic inhibits BMP6 induction of hepcidin expression in Hep3B cells. A and C, Hep3B cells were transfected with Hep-Luc and control Renilla luciferase vector (A) or mock-transfected (C) and were treated without (black bar) or with 5 ng/ml BMP6 (white bar) for 17 h, followed by measurement of relative luciferase activity (A) or HAMP relative to RPL19 mRNA by qRT-PCR (C). Results are reported as the mean fold change ± S.E. relative to untreated cells. B, Hep3B cells were transfected with Hep-Luc and control Renilla luciferase vector, in combination with increasing concentrations of miR-130a (gray bars) or negative control mimic (white bars) and were treated with 5 ng/ml BMP6 for 17 h, followed by measurement of relative luciferase activity. Results are reported as the mean fold change ± S.E. relative to negative control at each concentration. D, Hep3B cells were transfected with 3 or 10 nm miR-130a (gray bars) or negative control mimic (white bars) and were treated with 5 ng/ml BMP6 for 17 h, followed by measurement of HAMP relative to RPL19 mRNA by qRT-PCR. Results are reported as the mean fold change ± S.E. relative to the negative control at each concentration. For all panels, n = 3 experiments each performed in triplicate. **, p < 0.01, and ***, p < 0.001.