TABLE 1.
Primer sequences used for qRT-PCR analysis, cloning, or site-directed mutagenesis
F is forward, and R is reverse; h is human; m is mouse; and mut is mutant.
| Primer | Sequence (5′ to 3′) | Experimental method |
|---|---|---|
| hACVR1 F | tgccaaggggactggtgtaa | qRT-PCR |
| hACVR1 R | actgcgaacactacagagagaa | qRT-PCR |
| hHAMP F | cacaacagacgggacaac | qRT-PCR |
| hHAMP R | cgcagcagaaaatgcaga | qRT-PCR |
| hID1 F | gggattccactcgtgtgttt | qRT-PCR |
| hID1 R | ctgagaagcaccaaacgtga | qRT-PCR |
| hSMAD4 F | ctcatgtgatctatgcccgtc | qRT-PCR |
| hSMAD4 R | aggtgatacaactcgttcgtagt | qRT-PCR |
| hRPL19 F | tccgctgtggcaagaagaaggt | qRT-PCR |
| hRPPL9 R | accgtcacaggcttgcggat | qRT-PCR |
| mAcvr1 F | gtggaagattacaagccacca | qRT-PCR |
| mAcvr1 R | gggtctgagaaccatctgttagg | qRT-PCR |
| mBmp6 F | atggcaggactggatcattgc | qRT-PCR |
| mBmp6 R | ccatcacagtagttggcagcg | qRT-PCR |
| mHamp F | ttgcgataccaatgcagaaga | qRT-PCR |
| mHamp R | gatgtggctctaggctatgtt | qRT-PCR |
| mId1 F | ttggtctgtcggagcaaagcgt | qRT-PCR |
| mId1 R | cagccgttcatgtcgtagagca | qRT-PCR |
| mSmad4 F | aggtggcctgatctacacaag | qRT-PCR |
| mSmad4 R | acccgctcatagtgatatggatt | qRT-PCR |
| mRpl19 F | aggcatatgggcatagggaagag | qRT-PCR |
| mRpl19 R | ttgaccttcaggtacaggtgtg | qRT-PCR |
| hACVR1-UTR F | atcggccggcccattttcatagtgtcaagaa | Cloning |
| hACVR1-UTR R | actgtcgacgacttgaaaacagtttattt | Cloning |
| hSMAD4 BS1 F | ctagtctagagtatcttggggcaagactgcaaac | Cloning |
| hSMAD4 BS1-BS2 R | gcaggatgtcagaccctcaattctcgcagtagaagctgtgttcattccag | Cloning |
| hSMAD4 BS1-BS2 F | ctggaatgaacacagcttctactgcgagaattgagggtctgacatcctgc | Cloning |
| hSMAD4 BS2 R | atagtttaggccggccctgctgcaaggtagccaaggtc | Cloning |
| hACVR1-Mut1 F | cctgtgcttctcttctttattgttgcaggaattctttgcattcc | Mutagenesis |
| hACVR1-Mut1 R | ggaatgcaaagaattcctgcaacaataaagaagagaagcacagg | Mutagenesis |
| hACVR1-Mut2 F | gcactaggaattctttgcattccttacttgatgagttactcttaattttaaagacccaac | Mutagenesis |
| hACVR1-Mut2 R | gttgggtctttaaaattaagagtaactcatcaagtaaggaatgcaaagaattcctagtgc | Mutagenesis |
| hSMAD4 BS1-mut F | gattttttttttcttaacgtgatttgagtccaatctcagtgatg | Mutagenesis |
| hSMAD4 BS1-mut R | catcactgagattggactcaaatcacgttaagaaaaaaaaaatc | Mutagenesis |
| hSMAD4 BS2-mut F | tggctctgggttgggccagacagaacgtgactctagtttgccctctgc | Mutagenesis |
| hSMAD4 BS2-mut R | gcagagggcaaactagagtcacgttctgtctggcccaacccagagcca | Mutagenesis |