FIGURE 4.

Peptides without an optimal hydrophobic pocket 1 anchor residue compete for the binding to DR1 and replace other peptides. DR1 loaded with Alexa488-labeled CLIP (DR1-CLIP, 0.1 μm) was incubated without a peptide or with 5 μm of exchanging A1, A1L9, and WT, respectively, in the absence of DM (A) and in the presence of 0.4 μm DM (B). These data represent at least two independent experiments with three or four replicates each.