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Luminal calcium depletion causes COPII proteins to accumulate in enlarged, intensified peripheral puncta. NRK cells were CPA- or mock-treated and then fixed and immunolabeled for the endogenous VTC marker Rbet1 and the COPII subunit Sec31A (A) or Sec24C (B). Shown are single optical sections of deconvolved wide field fluorescence image stacks.
