FIGURE 7.

ALG-2/Sec31A interactions are required for p24 stability and targeting of p115. NRK cells were transfected with VSV-G-GFP along with ALG-2-RFP (A) or FLAG-sec31A (B) or with both (C–G). Cells were shifted to 32 °C for 12 min, fixed, and immunolabeled to detect FLAG-PRR and endogenous p24 (A–C), GPP130 (D), p58 (E), rbet1 (F), or p115 (G). Shown are single optical sections of deconvolved wide field image stacks. In each set of fields, green arrows are employed in the image plane that was merged as green and red arrows are used in the plane merged as red. White boxes indicate the area displayed in the merge if less than the entire field. Arrows in A, E, and F mark prominent cytoplasmic puncta decorated by ALG-2-RFP. Arrows in B–D, point out the Golgi area. The arrows in G mark regions of ER that co-localize with p115.