TABLE 1.
DQD/SDH family members included in amino acid sequence analysis and PCR primers used
For each poplar gene, a forward (F) and reverse (R) primer was used to amplify the complete open reading frame (start and stop codons are underlined).
| Name | Species | Sequence identifier (database) | Primers sequence (5′ to 3′) |
|---|---|---|---|
| EsccoAroE | E. coli | YP_006126142.1 (GenBankTM) | |
| EsccoAroD | E. coli | YP_006124567.1 (GenBankTM) | |
| EsccoYdiB | E. coli | YP_006124566.1 (GenBankTM) | |
| Arath | A. thaliana | NP_187286.1 (GenBankTM) | |
| Solly | S. lycopersicum | NP_001234051.1 (GenBankTM) | |
| Nicta1 | N. tabacum | AAS90325.1 (GenBankTM) | |
| Nicta2 | N. tabacum | AAS90324.2 (GenBankTM) | |
| Poptr1 | P. trichocarpa | Potri.010G019000.2 (Phytozome Version 3.0) | F, GGCTTAAUATGGATTCTGCAAGCAACGTCC |
| R, GGTTTAAUCTAGTACTTTGACATGATCTTCTGAAAGAGTTC | |||
| Poptr2 | P. trichocarpa | Potri.013G029900.2 (Phytozome Version 3.0) | F, GGCTTAAUATGGGGCGTGCTGGGATC |
| R, GGTTTAAUTCAGAATTTGGCTAGAACAATCTCCC | |||
| Poptr3 | P. trichocarpa | Potri.005G043400.1 (Phytozome Version 3.0) | F, GGCTTAAUATGGGGAGTGTTGGAGTCCTGAC |
| R, GGTTTAAUTCAGAATTTGGCTAAAACAATCTCCC | |||
| Poptr4 | P. trichocarpa | Potri.014G135500.3 (Phytozome Version 3.0) | F, GGCTTAAUATGGCATTCAAGAACAACCTCTTAG |
| R, GGTTTAAUTCAAAATTGCTCCAAGACAAGC | |||
| Poptr5 | P. trichocarpa | Potri.013G029800.1 (Phytozome Version 3.0) | F, GGCTTAAUATGGATCTCCAAAGCGCTG |
| R, GGTTTAAUTTATGTATTCCTTGCTAACACATCTCTAAT |