TABLE 3.
MIC and RT-PCR data for C. albicans isolates from candidiasis patients
| Patient no. | Isolate no. | MICa (μg/ml)
|
ΔCTb
|
Substitution(s) in Erg11p | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| POS | ITZ | FLZ | VOR | ACT1 | PMA1 | ERG11 | MDR1 | CDR1 | CDR2 | |||
| 1 | C482 | 0.25 | 0.5 | 16 | 0.5 | 7.9 | 7.5 | 10.4 | 13.7 | 9.2 | 9.4 | A107T |
| C486 | 0.25 | 1 | 16 | 0.25 | 8.0 | 8.1 | 9.8 | 12.3 | 11.2 | 10.1 | A107T | |
| 2 | C491 | 0.12 | 0.25 | 2 | 0.03 | 7.4 | 7.2 | 8.9 | 12.9 | 10.1 | 10.0 | D116E |
| C504 | 0.25 | 0.25 | 16 | 0.25 | 7.3 | 6.2 | 7.9 | 15.5 | 8.2 | 7.7 | F449S | |
| 3 | C498 | 0.008 | 0.06 | 1 | 0.008 | 7.4 | 5.4 | 9.6 | 12.6 | 8.2 | 10.8 | F145L, E266D, V488I |
| C502 | 0.008 | 0.06 | 1 | 0.008 | 7.2 | 5.9 | 7.4 | 11.7 | 10.6 | 10.8 | F145L, E266D, V488I | |
| C513 | 0.25 | 0.25 | 16 | 0.12 | 6.7 | 6.4 | 7.5 | 10.6 | 7.6 | 5.8 | F145L, E266D, V488I | |
| 4 | C527 | 0.12 | 0.5 | 256 | 4 | 7.5 | 6.6 | 9.1 | 12.6 | 8.9 | 8.6 | Y132H, G450E |
| C528 | 0.12 | 0.5 | 256 | 4 | 7.3 | 5.9 | 9.0 | 11.7 | 7.7 | 7.7 | Y132H, G450E | |
| C530 | 0.12 | 0.25 | 256 | 0.5 | 7.0 | 6.3 | 8.5 | 12.0 | 8.8 | 9.9 | G450E, G464S | |
| C534 | 0.12 | 0.5 | 256 | 4 | 7.3 | 6.4 | 8.1 | 11.0 | 8.3 | 8.8 | Y132H, G450E | |
| C546 | 0.12 | 0.25 | 128 | 2 | 8.1 | 7.5 | 9.6 | 11.9 | 9.4 | 9.4 | Y132H, G450E | |
| C536 | 0.25 | 0.5 | 256 | 4 | 8.2 | 7.7 | 9.8 | 11.7 | 9.2 | 9.4 | Y132H, G450E | |
| C572 | 0.25 | 0.25 | >256 | 8 | 8.0 | 7.4 | 9.5 | 12.1 | 9.5 | 9.2 | Y132H, G450E | |
| 5 | C481 | 0.25 | 0.5 | 32 | 0.5 | 7.9 | 7.2 | 9.9 | 15.7 | 9.4 | 9.0 | F128T, F145L |
| C483 | 0.25 | 0.5 | 8 | 0.12 | 8.2 | 7.3 | 11.0 | 14.8 | 10.0 | 9.5 | F145L | |
| 6 | C535 | 0.5 | 2 | 256 | 8 | 7.7 | 7.1 | 9.8 | 9.0 | 9.6 | 10.9 | Y132H, G448V |
| C539 | 0.25 | 2 | 128 | 8 | 7.2 | 6.8 | 11.0 | 8.3 | 6.6 | 7.1 | Y132H, G448V | |
| 7 | C516 | 0.12 | 0.25 | 32 | 0.5 | 7.3 | 7.1 | 9.8 | 14.0 | 9.7 | 9.7 | K128T, Y132F, F145L |
| 8 | C470 | 0.5 | 0.5 | 32 | 0.25 | 7.3 | 7.0 | 9.0 | 13.5 | 8.7 | 8.5 | S405F |
| C478 | 0.5 | 0.5 | 64 | 0.5 | 7.6 | 6.9 | 8.6 | 12.6 | 9.1 | 8.5 | S405F | |
| 9 | C490 | 0.5 | 1 | 32 | 0.25 | 8.3 | 7.8 | 10.6 | 9.4 | 9.0 | 9.2 | V452A |
| C497 | 0.5 | 2 | 64 | 0.5 | 8.4 | 7.7 | 9.1 | 12.5 | 10.0 | 9.3 | K128T, V452A | |
| 10 | C526 | 0.5 | 1 | 32 | 0.5 | 7.8 | 7.1 | 9.6 | 13.6 | 9.2 | 9.2 | V509M |
| C533 | 0.5 | 0.5 | 32 | 1 | 7.9 | 7.4 | 9.8 | 13.6 | 9.3 | 9.9 | V509M | |
| 11 | C594 | 0.5 | 2 | 128 | 16 | 7.2 | 6.5 | 9.0 | 11.3 | 8.2 | 7.5 | Y132H, S405F |
| C600 | 1 | 4 | >256 | 16 | 7.7 | 7.1 | 8.5 | 12.3 | 9.4 | 8.2 | Y132H, S405F | |
| 12 | C577 | 0.06 | 0.06 | 128 | 0.5 | 6.5 | 5.0 | 9.0 | 5.3 | 8.1 | 12.9 | G464S |
| C583 | 0.25 | 0.25 | 256 | 1 | 6.8 | 5.5 | 9.4 | 5.7 | 6.9 | 10.7 | G464S | |
| C587 | 0.5 | 0.5 | 256 | 4 | 7.7 | 7.3 | 9.2 | 8.2 | 10.2 | 9.6 | G464S | |
| 13 | C480 | 0.03 | 0.12 | 128 | 8 | 8.3 | 7.4 | 9.3 | 9.2 | 12.2 | 15.1 | K128T, G464S, R467I |
| 14 | C438 | 0.5 | 0.5 | 128 | 2 | 6.6 | 6.0 | 7.9 | 13.3 | 7.1 | 7.5 | Y257H, G464S |
| C439 | 1 | 4 | >256 | 16 | 7.1 | 6.2 | 7.6 | 13.5 | 7.7 | 7.5 | Y257H, G464S, G307S | |
| C440 | 4 | 8 | >256 | >16 | 7.3 | 6.4 | 8.0 | 13.5 | 7.9 | 7.7 | Y257H, G464S, G307S, A61V | |
| 15 | C444 | 0.5 | 0.5 | 256 | 0.5 | 7.0 | 5.8 | 7.4 | 13.2 | 7.8 | 7.8 | K143R |
| 16 | C489 | 0.25 | 0.25 | 128 | 1 | 7.2 | 6.1 | 8.8 | 12.8 | 10.1 | 9.5 | Y132H, Y257H, E266D |
| 17 | C477 | 1 | 0.25 | 256 | 16 | 7.6 | 6.1 | 9.3 | 7.3 | 10.5 | 15.3 | K128T, G464S, R467I |
| 18 | C507 | 1 | 2 | 64 | 8 | 6.1 | 7.2 | 8.6 | 10.5 | 8.3 | 8.4 | Y132H, H283R, G464S |
a
See Table 2, footnote a.
b
Values that fall outside the 3-SD range measured in the azole-susceptible isolates (see text for details) are shown in boldface italic.