TABLE 5.
Changes in azole susceptibility resulting from expressing C. albicans ERG11 alleles in S. cerevisiae
| Original C. albicans isolate | Substitution(s) in expressed C. albicans Erg11p | MICa (μg/ml)
|
|||
|---|---|---|---|---|---|
| POS | ITZ | FLZ | VOR | ||
| None | None (vector alone) | 0.06 | 0.125 | 4 | 0.016 |
| C43 | None (wild type) | 0.06 | 0.5 | 16 | 0.125 |
| C441 | K143R | 0.25 | 1 | >256 | 1 |
| C587 | G464S | 0.25 | 1 | >256 | 1 |
| C477 | K128T, G464S, R467I | 0.125 | 0.5 | 32 | 0.25 |
| C530 | G450E, G464S | 1 | 1 | 128 | 1 |
| C600 | Y132H, S405F | 0.5 | >8 | >256 | 16 |
| C535 | Y132H, G448V | 0.25 | 1 | >256 | 0.03 |
| C572 | Y132H, G450E | 0.5 | 2 | >256 | 8 |
| C438 | Y257H, G464S | 0.5 | 1 | 128 | 4 |
| C439 | Y257H, G464S, G307S | 1 | 1 | >256 | 8 |
| C440 | Y257H, G464S, G307S, A61V | 4 | 8 | >256 | 16 |
| NAb | A61V | 0.25 | 0.5 | 16 | 0.125 |
a
See Table 2, footnote a.
b
NA, not applicable; mutation was engineered into a wild-type ERG11 gene.