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. 2014 Mar 31;210(6):904–912. doi: 10.1093/infdis/jiu194

Figure 4.

Figure 4.

Oxidative stress level was elevated in the striatum of simian immunodeficiency virus (SIV)–infected macaques and correlated with monoamine oxidase (MAO) B messenger RNA (mRNA) level. A, Reduced and oxidized glutathione (GSH and GSSG, respectively) were measured by a commercially available fluorimetric method. SIV-infected macaques had lower GSH/GSSG ratios than uninfected macaques (P < .001), which is indicative of oxidative stress. B, mRNA levels of genes involved in reactive oxygen species (ROS) detoxification (CAT, GPX1, GPX3, GPX4, GPX7, GSR, GSS, PRDX1, PRDX2, PRDX3, PRDX4, PRDX6, SRXN1, TXN, TXNRD1, TXNRD1, SOD1, SOD2, SOD3, and CCS) were measured using a custom Nanostring gene expression assay. To assess the cumulative impact on genes involved in ROS detoxification, the geometric mean of the counts of these transcripts was calculated and compared between SIV-negative and SIV-positive macaques. Transcripts of genes involved in ROS detoxification were elevated in SIV-infected macaques, compared with those in uninfected controls (P = .007). C, MAO B mRNA level, as measured by Nanostring analysis, negatively correlated with the ratio of GSH/GSSG (P = .044). D, MAO B mRNA positively correlated with the geometric mean counts of transcripts involved in ROS detoxification (P < .001). Bars represent medians. Statistical analyses used the Mann–Whitney test (A and B) or Spearman correlation (C and D).