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. Author manuscript; available in PMC: 2014 Sep 5.
Published in final edited form as: Nitric Oxide. 2012 Jun 15;27(3):150–160. doi: 10.1016/j.niox.2012.06.003

Figure 1. GSNO production from nanoparticles.

Figure 1

Figure 1

Figure 1

NO-np (A), SNO-np (B), and NAC-SNO-np-2 (C) were incubated at 20 mg/ml in 0.5 mM DTPA/PBS, pH 7.4 for 1 hour at room temperature shielded from light in the absence and presence of GSH (20 mM) while mixing on a lab rotator. Supernatants were diluted (100x for extracts and 50x for GSH-reaction mixtures) and analyzed by RPHPLC as described in the text. GSH-reaction mixtures of all the particles displayed a peak corresponding to GSNO, which was absent in the respective extracts (in the absence of GSH). Peaks identities are as follows: 1 = GSH, 2 = nitrite, 3 = GSSG, 3a = nitrate, 4 = GSNO, 5 = non-characterized oxidized product of NAC-SNO, 6 = NAC-SNO.