TABLE 1.
Salmonella strains and plasmids used in this study
Strain or plasmid | Characteristic | Origin or reference |
---|---|---|
S. enterica serovar Typhimurium | ||
14028s | Wild type | ATCCa |
14028ΔramA | ramA null mutant | 10 |
E. coli | ||
MC1061 | Wild type | ATCC |
AG100 | Wild type | 6 |
AG100kana | ΔmarA | 6 |
Plasmids | ||
pBluescript | Cloning vector | Stratagene |
pB1-ramA | Constitutive RamA expression | 10 |
pTrcHisA | IPTG-inducible plasmid | Invitrogen |
pTrcHisA-ramA | Inducible RamA expression | 10 |
ATCC, American Type Culture Collection.