Table II.
Primer sequences and annealing temperatures used for the methylation-specific polymerase chain reaction.
| Primer | Sequence, 5′-3′ | Annealing temperature, °C | Product size, bp | Reference |
|---|---|---|---|---|
| RNF180 MF | GGAGAAAAATTTTTTTACGGTTTC | 50 | 109 | |
| RNF180 MR | CACGTCTACGAATTCCCAC | |||
| RNF180 UF | AGGGAGAAAAATTTTTTTATGGTTTT | 46 | 109 | |
| RNF180 UR | CACATCTACAAATTCCCACCC | |||
| DAPK1 MF | GGATAGTCGGATCGAGTTAACGTC | 52 | 98 | (14) |
| DAPK1 MR | CCCTCCCAAACGCCGA | |||
| DAPK1 UF | GGAGGATAGTTGGATTGAGTTAATGTT | 56 | 106 | |
| DAPK1 UR | CAAATCCCTCCCAAACACCAA | |||
| SFRP2 MF | GGGTCGGAGTTTTTCGGAGTTGCGC | 58 | 138 | (13) |
| SFRP2 MR | CCGCTCTCTTCGCTAAATACGACTCG | |||
| SFRP2 UF | TTTTGGGTTGGAGTTTTTTGGAGTTGTGT | 54 | 145 | |
| SFRP2 UR | AACCCACTCTCTTCACTAAATACAACTCA |
RNF180, ring finger protein 180; DAPK1, death-associated protein kinase 1; SFRP2, secreted frizzled-related protein 2; MF, methylated forward; MR, methylated reverse; UF, unmethylated forward, UR, unmethylated reverse; bp, base pair.