(A) Relative expression of miR-449a in gastric carcinoma and tumor-adjacent normal tissues, as determined by qPCR. ‘a’ represents the tumor-adjacent normal tissue; relative expression, 24.665±1.557. ‘b’ represents the gastric carcinoma tissue; relative expression, 49.207±13.433, P<0.001. (B) The relative expression of the CDK6 protein in the gastric carcinoma and tumor-adjacent normal tissues, as determined by western blot analysis. (C) ‘a’ represent the tumor-adjacent normal tissue; relative expression, 0.482±0.290. ‘b’ represents the gastric carcinoma tissue; relative expression, 0.792±0.165, P=0.003. (D) The relative expression of CDK6 protein was negatively correlated with the relative expression of miR-449a, as determined by Spearman’s correlation analysis, r(tumor-adjacent normal tissue)=-0.614; r(gastric carcinoma tissue)=-0.694, P<0.001. Relative expression is measured as a percentage and *P<0.05 vs. tumor-adjacent normal tissues.. miR, microRNA; qPCR, quantitative PCR; CDK6, cyclin-dependent kinase 6.