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. 2014 Aug 1;8(4):1889–1895. doi: 10.3892/ol.2014.2404

Figure 2.

Figure 2

Expression levels of candidate reference genes in benign plural effusion (BPE) and lung adenocarcinoma-associated malignant pleural effusion (LA-MPE) samples. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analyses of 46 BPE and 45 LA-MPE samples were performed. (A) Box-and-whisker plot of the primary Cq values of U6 snRNA and five candidate reference miRNAs (miR-192, miR-20a, miR-221, miR-222 and miR-16)in the 91 pleural effusion samples. (B) Box-and-whisker plot of the Cq values of candidate reference genes in the BPE and LA-MPE samples. The expression levels of all candidate reference genes did not differ significantly between BPE and LA-MPE samples (P>0.05). The Cq values were corrected to PCR efficiency and two inter-plate controls. The boxes (light gray, BPE and dark gray, LA-MPE) signify the upper and lower quartiles, the median is indicated by a horizontal line, and the whiskers depict the 10th and 90th percentiles. The P-values of the Mann-Whiney U tests are indicated above each plot.