Table III.
Ranking and best combination of candidate reference genes in pleural effusion samples as determined by the expression stability values calculated using NormFinder and BestKeeper.
| NormFinder | BestKeeper | |||
|---|---|---|---|---|
|
|
|
|||
| Rank | Gene | Stability valuea | Gene | Stability valuea |
| 1 | U6 snRNA | 0.096 | miR-192 | 0.160 |
| 2 | miR-192 | 0.103 | U6 snRNA | 0.177 |
| 3 | miR-20a | 0.158 | miR-20a | 0.219 |
| 4 | miR-221 | 0.185 | miR-222 | 0.222 |
| 5 | miR-222 | 0.198 | miR-221 | 0.267 |
| 6 | miR-16 | 0.251 | miR-16 | 0.374 |
| Best combination | U6 snRNA/miR-192 | 0.081 | - | - |
a
High expression stability is indicated by a low stability value.
snRNA, small nuclear RNA; miRNA, microRNA.